See below Description Glycyrrhiza glabra , or Licorice is a flowering, herbaceous perennial legume native to Europe, parts of Asia, and most of North America, but not to the Southeastern United States.
Diseases, Insects, and Other Plant Problems: Pests and diseases include: spider mites, slugs, snails, powdery mildew, and rust. Licorice extract or oil is also used in foods, beverages, cosmetics, and toothpaste.
It used in Ayurvedic holistic medicine to enhance the immune system, treat peptic ulcers, as an expectorant, a liver enzyme stimulant, a laxative, and a diuretic. Edibility: Extracts and oils of the root of this plant are used in candies and as a sweetener in foods and tobacco products. Flowers are light pink to light blue in color. Eom, and Y. Iranian Journal of Basic Medical Sciences, vol. Basar, O. Oridupa, K. Ritchie et al. See, J. Kim, G.
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Bisogni, G. Rossi, and L. Li, R. Sun, and R. Pharmacological Research, vol. Celik, A. Karakus, C. Zeren et al. Log in with Facebook Log in with Google. Remember me on this computer. Enter the email address you signed up with and we'll email you a reset link. Need an account? Click here to sign up. Download Free PDF. Micropropagation of licorice Glycyrrhiza glabra L. Yaser Hassan Dewir. A short summary of this paper. Box , Riyadh , Saudi Arabia. Received: 3 November ; Accepted: 4 February ; doi The aim of the present study was to determine the optimal nodal explant type terminal, intermediate and basal and cytokinins 6-benzyladenine and thidiazuron [TDZ] at concentrations of 0 to 3 mg L-1 for bud break and axillary shoot proliferation of Glycyrrhiza glabra L.
The intermediate nodal explants were effective for the establishment of G. The regenerated plantlets were acclimatized, with a survival rate of Thus, the high percentage of bud break and rate of shoot proliferation and successful acclimatization established a simple and efficient micropropagation of G.
Key words: Acclimatization, Fabaceae, rooting, shoot proliferation, tissue browning. The leaves are multifoliate with pairs of leaflets. The flowers are papilionaceous, spikes are lavender to violet in color, and the fruit is a compressed pod containing seeds Sofia and Walter, The roots and rhizomes, which have a cylindrical shape and diameter of 0.
Glycyrrhiza glabra has been reported to possess several medicinal properties such as antidiabetic, anti-inflammatory, antiulcer, antiallergic, anticarcinogenic, laxative, and antipyretic activities Brown ; Badkhane et al.
This plant occupies the land for an average period of yr, with a maximal yield of 2 t roots per acre Vispute and Khopade, The demand for G. Conventional propagation of G. Moreover, a large number of stock plants need to be maintained. Seeds are also used for propagation of G.
In vitro propagation can be used to ensure the supply of planting material for G. Previous studies on in vitro propagation of G. Direct regeneration of G. However, limited studies highlighted the influence of the type of nodal explants position on the main stem on culture establishment of G. Composition of the media and content of plant growth regulators PGRs play a crucial role for optimal growth and morphogenesis of G. Moreover, the success of in vitro regeneration and propagation depends on a series of stages with specific requirements for each step.
In this study, we aimed to improve micropropagation of G. Young, fresh and healthy growing shoots were collected and used as donor for initial explant material for initiation of aseptic culture.
The young shoots were cut into nodal explants cm and classified as terminal 1st and 2nd node , intermediate 3rd to 5th node , and basal 6th to 8th node nodal explants with relation to their position from the shoot apex Figure 1. Then, the explants were washed three times with sterile distilled water, treated with 0. The aseptic and healthy nodal explants were used for the shoot proliferation experiment.
Bud break and axillary shoot proliferation The nodal explants from the intermediate regions 3rd to 5th node were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine BAP; 0, 1, 2, and 3 mg L-1 and thidiazuron TDZ; 0, 1, 2, and 3 mg L-1 or combination of BAP and TDZ at 1 and 2 mg L-1, respectively. Shoot growth and proliferation responses in terms of percentage of bud break, number of axillary shoots, and fresh and dry weight of whole explants were recorded after 30 d culture.
In vitro rooting and culture conditions Axillary shoots of G. Rooting percentage, time to root initiation, number of leaves, root length and number of roots, and plant height were recorded after 30 d culture.
Type of nodal explants used for culture initiation of Glycyrrhiza glabra. Terminal node explant 1st and 2nd nodes , intermediate node explant 3rd to 5th nodes and basal node explant 6th to 8th node. The PGRs were added prior to autoclaving. The pH of the medium was adjusted to 5. Acclimatization of the regenerated plantlet The regenerated plantlets were transferred to pots filled with different combinations of growing media, i.
The plantlets were maintained at high humidity by covering with transparent polythene bags to ensure high humidity. Moreover, reduction in testicular tissue immunoreactivity to Bclassociated X protein and cyclogenase In conclusion, licorice extract mitigated the toxic effects of MTX-induced testicular dysfunction at biochemical, molecular, and cellular levels. Keywords: gene expression; licorice extract; methotrexate; oxidative stress; testis dysfunction.
Published by Oxford University Press.
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